We narrowed to 10,106 results for: UTY
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Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
ExpressionPlantPromoterCmYLCV PromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-ZNF354C_mutant_M41K-L42K-E43K
Plasmid#194185PurposeExpresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)DepositorInsertZNF354C (ZNF354C Human)
TagsMyc-DDKExpressionMammalianMutationKRAB domain mutant M41K/L42K/E43KPromoterCMVAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
iEscSnFR
Plasmid#182807PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR
Tags6xHis, HA and MycExpressionBacterialPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
iFluoxSnFR
Plasmid#182808PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR
Tags6xHis, HA and MycExpressionBacterialPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)
Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla–insM
Plasmid#191226PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationremoved methionine at 122 in the protein sequencePromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla+N122D–insM
Plasmid#191227PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationremoved methionine at 122 and replaced asparagine…PromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
ExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjT-tdTomato-PGK-Neo
Plasmid#186173PurposeKnock-in gene targeting in marmoset cells at TBXT locusDepositorInserttdTomato
UseMarmoset targetingAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-tdTomato-PGK-Neo
Plasmid#186172PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserttdTomato
UseMarmoset targetingAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-hCD271-PGK-Neo
Plasmid#186171PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserthCD271
UseMarmoset targetingMutationhuman codon-optimizedAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-EGFP-PGK-Puro
Plasmid#186167PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertEGFP
UseMarmoset targetingAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-1
Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)
Plasmid#166423PurposeExpress pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)DepositorInsertPythium oligandrum PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)
Plasmid#166424PurposeExpress pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)DepositorInsertReticulomyxa filosa PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)
Plasmid#166425PurposeExpress pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)DepositorInsertSphaeforma arctica PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Drosophila melanogaster CBS-pair domain
Plasmid#166436PurposeExpress pGEX-6P-1 Drosophila melanogaster CBS-pair domainDepositorInsertDrosophila melanogaster CBS-pair domain (Cbs Fly)
TagsGSTExpressionBacterialPromoterTac, LacI, AmpAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
Plasmid#166415PurposeExpress pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)DepositorInsertAcytostelium subglobosum PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)
Plasmid#166416PurposeExpress pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)DepositorInsertcaenorhabditis elegans PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)
Plasmid#166419PurposeExpress pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)DepositorInsertGaldieria sulphuraria PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)
Plasmid#166420PurposeExpress pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)DepositorInsertMonosiga brevicollis PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2
Plasmid#178969PurposeExpresses miRFP2 in worm cellsDepositorInsertwmiRFP2
TagsnoExpressionWormMutationNOPromotertag-168 promoterAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptia1l-hsp-GFP
Plasmid#177837PurposeDonor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9DepositorInserttia1l-sgRNA target site-hsp70-EGFP
UseCRISPRTagsEGFPPromoterhsp70Available SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+7U
Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_mouse-caspase-11-CARD+canine-Caspase-1/4-catalytic domain
Plasmid#183398PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-11 fused to catalytic domain of canine caspase-1/4
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-Caspase-1-H340D
Plasmid#183360PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-caspase-1/4b
Plasmid#183394PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of human caspase-1 fused to human caspase-4 (CASP1 Synthetic, Human)
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB
Plasmid#180197PurposeExpresses FLAG-tagged Capsaspora NF-kB protein in mammalian cellsDepositorInsertCo-NF-kB
TagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-1224PromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-llo190-mc38minor
Plasmid#174604Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1DepositorInsertpalmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj13
Plasmid#173143PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj13 (kcnj13 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj15
Plasmid#173145PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj15 (kcnj15 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj3a
Plasmid#173129PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj3a (kcnj3a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj5
Plasmid#173132PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj5 (kcnj5 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj6
Plasmid#173133PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj6 (kcnj6 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-V9
Plasmid#173797PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
ExpressionBacterial and PlantAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-V7
Plasmid#173796PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
ExpressionBacterial and PlantAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-V6
Plasmid#173795PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
ExpressionBacterial and PlantAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-V4
Plasmid#173794PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
ExpressionBacterial and PlantAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
Tags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHM019Env
Plasmid#137685PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM073Env
Plasmid#137669PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM045Env
Plasmid#137687PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM043Env
Plasmid#137675PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM040Env
Plasmid#137677PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only