We narrowed to 5,560 results for: Chia

1. pDsEndoNA2

   Plasmid

   #174728

   Purpose

   Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

   Insert

   DsRed-Monomer
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAJM.715

   Plasmid

   #108516

   Purpose

   PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PTac-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTJV1ScKm-rpoB

   Plasmid

   #166980

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   NeoR/KanR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet28a-ClbB-NRPS-NHis

   Plasmid

   #49217

   Purpose

   NRPS module of ClbB containing CAT domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbB-NRPS
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa4-1080 of reference sequence NP_754362…

   Promoter

   T7

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCH1 - pBait-empty

   Plasmid

   #174663

   Purpose

   arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMDICI

   Plasmid

   #51652

   Purpose

   E coli genetic modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   CmR
   

   Use

   Genetic modification

   Tags

   Expression

   Mutation

   Promoter

   unknown

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-QUASM1

    Plasmid

    #155114

    Purpose

    5' entry vector containing QUASM1 promoter element.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1
    

    Use

    Gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-3a-chpBK

    Plasmid

    #155201

    Purpose

    To express recombinant ChpBK protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Inserts

    chpS-chpBK-6×His-Tag
    GyrA-CBD-10xHis-Tag
    

    Use

    Tags

    10xHis and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-6xHis-BglII

    Plasmid

    #196654

    Purpose

    Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.717

    Plasmid

    #108517

    Purpose

    PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTet*-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAJM.712

    Plasmid

    #108513

    Purpose

    PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PCymRC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAJM.716

    Plasmid

    #108518

    Purpose

    PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PBAD-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAJM.714

    Plasmid

    #108515

    Purpose

    PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PVanCC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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