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MS2-adRNA (RAB7A-GAC site)

Plasmid

#170140

Purpose

AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-RAB7A(GAC)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1(W795R)-StayGold

Plasmid

#210822

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

Tags

StayGold

Expression

Mammalian

Mutation

Changed Trp-795 to Arg

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-TwinStrep-His

Plasmid

#210816

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

Tags

StayGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nmba

Plasmid

#184821

Purpose

Used to synthesize RNA probe for in situ hybridization for nmba

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nmba (nmba Zebrafish)

Use

Template for synthesis of nmba probe

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1 PST deletion

Plasmid

#207109

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 PST deletion

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1 BRCT domain deletion

Plasmid

#207110

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 BRCT domain deletion

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1161

Plasmid

#217964

Purpose

For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3.1 (H3C1 Human)

Use

Tags

eGFP, APEX

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1313

Plasmid

#217955

Purpose

Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3.1 (H3C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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α-synuclein-3H

Plasmid

#205715

Purpose

Mammalian expression of alpha-synuclein-3H

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-αS-2

Plasmid

#205706

Purpose

Mammalian expression of alpha-synuclein tandem dimer

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein tandem dimer (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pETM11-6xHis-TEV-hORF1p_StammerAAA

Plasmid

#202587

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Use

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab12 Q101L

Plasmid

#207347

Purpose

Bacterial expression of Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

Rab12 (RAB12 Human)

Use

Tags

2xHis

Expression

Bacterial

Mutation

Q101L

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-N.Txnip

Plasmid

#206348

Purpose

AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

N terminal half (1-228aa)

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sC.Txnip

Plasmid

#206351

Purpose

AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

Short C terminal half (255-397aa)

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-nt.Txnip(C247S)(1-320aa)

Plasmid

#206352

Purpose

AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

(1-320aa), C247S

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-dnHIF

Plasmid

#206343

Purpose

AAV plasmid expressing dominant negative HIF1A in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

dominant negative hif1a

Use

AAV

Tags

Expression

Mutation

dominant negative HIF1A

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-nt.Txnip(C247S)(1-301aa)

Plasmid

#206354

Purpose

AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

(1-301aa), C247S

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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