We narrowed to 5,560 results for: chia

1. pCas9CR5-NG

   Plasmid

   #120228

   Purpose

   pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGAPZ-STE2-1D4

   Plasmid

   #133423

   Purpose

   Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C59S, C252A. No alpha factor secretion signal in …

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9CR4-NG

   Plasmid

   #120225

   Purpose

   pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pet15_rbsk

   Plasmid

   #220227

   Purpose

   Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   ribokinase (rbsK E.coli)
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHflu2

   Plasmid

   #203880

   Purpose

   pHI1 with insertion of ampicillin-resistance gene

   Depositor

   Bogumil Karas

   Insert

   broad-spectrum class A beta-lactamase TEM-116
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

   Plasmid

   #177231

   Purpose

   Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgNeo1/sgNT1/sgNeo2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pxCas9CR4

   Plasmid

   #111656

   Purpose

   PCas9CR4 with the xCas9 mutations allowing NG PAM sites

   Depositor

   Christopher Reisch

   Insert

   TetR and xCas9
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   xCas9 mutations

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Ec holA(Y316A)-FLAG-pET(11a)(ACH-)

   Plasmid

   #238346

   Purpose

   over-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coli

   Depositor

   Michael O'Donnell

   Insert

   C-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)
   

   Use

   Tags

   3X FLAG

   Expression

   Bacterial

   Mutation

   Y316A

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ec holB-pET(11a)(ACH-)

   Plasmid

   #237234

   Purpose

   over-express E. coli holB (Pol III delta prime subunit)

   Depositor

   Michael O'Donnell

   Insert

   holB (delta prime) (holB E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAIP4_3D1B

    Plasmid

    #234230

    Purpose

    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3D1B (tas3 Fission Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAIP4_5F66

    Plasmid

    #234226

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5F66 (PPIA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAIP4_3TOW

    Plasmid

    #234201

    Purpose

    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3TOW (MVB12B Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAIP4_5EIP

    Plasmid

    #234188

    Purpose

    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5EIP (mmi1 Fission Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAIP4_5KUW

    Plasmid

    #234183

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5KUW (PPIA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAIP4_3ONH

    Plasmid

    #234179

    Purpose

    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3ONH (UBA2 Budding Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_1EYH

    Plasmid

    #234159

    Purpose

    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1EYH (Epn1 Rat)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_6BIO

    Plasmid

    #234152

    Purpose

    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6BIO (TVAG_298080 Trichomonas vaginalis)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGEX-N-TEV-Twin-Strep

    Plasmid

    #222014

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

    Depositor

    Chiaki Murakami

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas9CR5

    Plasmid

    #120227

    Purpose

    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag

    Depositor

    Christopher Reisch

    Insert

    SpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Stop codon inserted before ssrA degradation tag

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHL 1915

    Plasmid

    #53022

    Purpose

    AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)

    Depositor

    Han Lim

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and fimS fusion

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pBN98

    Plasmid

    #35133

    Depositor

    John McCusker

    Article

    Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

    Insert

    kanMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

25. PIA586

    Plasmid

    #104399

    Purpose

    vector for expression of single E.coli RNAP subunit

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoD
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKSJ340

    Plasmid

    #27125

    Depositor

    Alan Finkelstein

    Article

    Jakes et al Mol Microbiol. 2010 Feb;75(3):567-78. do

    Insert

    Colicin Ia + Ia immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pAdEasy 2-GFP beta-gal

    Plasmid

    #16407

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

28. FLIPrbs

    Plasmid

    #17860

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    D208G

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMKV006

    Plasmid

    #35109

    Depositor

    John McCusker

    Article

    Vorachek-Warren et al Yeast. 2004 Jan 30;21(2):163-71.

    Insert

    loxp-dsdaMX4-loxp
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PsbA2-HMGS-HMGR-ATOB-NPTI

    Plasmid

    #73338

    Purpose

    Construct expressing the upper MVA pathway in Synechocystis PCC 6803

    Depositor

    Anastasios Melis

    Insert

    HMGS-HMGR-ATOB-NPTI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    psbA2

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FtsZ-Dendra2

    Plasmid

    #92038

    Purpose

    Express FtsZ-Dendra2

    Depositor

    Ji Yu

    Article

    Niu et al Biophys J. 2008 Aug;95(4):2009-16. doi:

    Insert

    FtsZ
    

    Use

    Tags

    Dendra2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 5407 pClpB

    Plasmid

    #1235

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    ClpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHL 636

    Plasmid

    #53015

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Inserts

    fimE
    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMKV001

    Plasmid

    #35107

    Depositor

    John McCusker

    Article

    Vorachek-Warren et al Yeast. 2004 Jan 30;21(2):163-71.

    Insert

    dsdaMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBADmod1-linker-deGFP

    Plasmid

    #45397

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Ec FkP-dnaN-pRSFDuet

    Plasmid

    #238342

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta(wt) in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (wt) (dnaN E. coli)
    

    Use

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    none (wt)

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAIP4_6IYE

    Plasmid

    #234237

    Purpose

    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pAIP4_4IPC

    Plasmid

    #234229

    Purpose

    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4IPC (RPA1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pAIP4_3CX2

    Plasmid

    #234221

    Purpose

    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3CX2 (MYBPC3 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge