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Showing: 3681 - 3720 of 17142 results

  5. MSCV-N-Flag-HA-GFP

    Plasmid
    #41034
    Purpose
    Retroviral expression of GFP in mammalian cells
    Depositor
    Wade Harper
    Article
    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.
    Insert
    GFP
    Use
    Retroviral; Gateway destination vector
    Tags
    Flag, HA, and IRES Puro
    Expression
    Mammalian
    Mutation
    Promoter
    LTR-driven expression
    Available since
    Oct. 26, 2012
    Availability
    Academic Institutions and Nonprofits only

  7. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  8. pCRUSH

    Plasmid
    #54480
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pBABE

    Plasmid
    #21836
    Depositor
    David Ron
    Insert
    EGFP
    Use
    Retroviral
    Tags
    farnysylation signal
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21836-plasmid-map-sequence-id-10029

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  22. mCherry-Dyskerin

    Plasmid
    #207577
    Purpose
    N-terminal mCherry-tag using SalI/EcoRI
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207577-plasmid-map-sequence-id-429490

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  23. pOP004

    Plasmid
    #185985
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185985-plasmid-map-sequence-id-378299

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  24. pOP005

    Plasmid
    #186030
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186030-plasmid-map-sequence-id-371540

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  33. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  35. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  36. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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Showing: 3681 - 3720 of 17142 results