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Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-tdTomato-SV40pA-FKF
Plasmid#119867PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF2 pBacPAK8
Plasmid#15326DepositorInsertELL Associated Factor 2 (EAF2 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG103QDProCFPp303
Plasmid#15591DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMHBAD14
Plasmid#42304DepositorInsertNpuDnaE C-intein
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHDV
Plasmid#51058PurposeSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK234_027
Plasmid#123907PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL 25Q+ProCFPp303
Plasmid#15574DepositorInserthtt25Q + Pro (HTT Human)
UseTagsCFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-SZT2 2241-end
Plasmid#87039Purposemammalian expression of SZT2 fragmentDepositorInsertSZT2 (SZT2 Human)
UseTagsFlagExpressionMammalianMutationAA 2241-endPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-SZT2 1-1021
Plasmid#87036Purposemammalian expression of SZT2 fragmentDepositorInsertSZT2 (SZT2 Human)
UseTagsFlagExpressionMammalianMutationAA 1-1021PromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK0_001
Plasmid#123924PurposeEncodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK systemDepositorInsertTU-sgRNA - L1/RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_055
Plasmid#123920PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL HA25QDProCFPp303
Plasmid#15578DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL HA103QDProCFPp303
Plasmid#15579DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
MTK0_049
Plasmid#123946PurposeEncodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK systemDepositorInserthThal5.10-2_hg18 landing pad destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC20-Tpm4.2 (Rn)
Plasmid#99359PurposeBacterial expression vector containing cDNA encoding for the Rattus norvegicus tropomyosin, Tpm4.2 under the control of the pRhamnose promoterDepositorInsertTpm4.2 (Tpm4 Rat)
UseTagsExpressionBacterialMutationPromoterRhamnoseAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H02
Plasmid#70956PurposeGateway entry cloneDepositorInsertIswi (Iswi Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only