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Plasmid#91893PurposeMammalian expression of mouse Gfi1DepositorInsertGfi1 (Gfi1 Mouse)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1396A
Plasmid#188126PurposeExpresses C-terminal flag-tagged human CAD S1396A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Cdk6 R31C-IRES-EGFP
Plasmid#153946PurposepiggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFPDepositorInsertCdk6 (Cdk6 Mouse)
UseTagsExpressionMammalianMutationR31CPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)
Plasmid#64314Purposechondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cellsDepositorUseLentiviralTagsEGFP and IRES-PUROExpressionMammalianMutationN/APromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Gfi1:FLAG-IRES-EGFP
Plasmid#153945PurposepiggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFPDepositorInsertGfi1 (Gfi1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSC-ASCL1-IRES-GFP-T2A-Sox11
Plasmid#182233PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189DepositorInsertASCL1-IRES-GFP-T2A-Sox11
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chx10 (3kb proximal 5' region)-GFP-IRES-AP
Plasmid#18815DepositorInsertChx10 (3kb proximal 5' region)-GFP-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88 (S34Y)-CpLxIS
Plasmid#131349PurposeExpresses MyD88 S34Y with 2 copies of pLxIS motif from STING fused to its C-terminusDepositorInsertMyD88 (S34Y)-CpLxIS (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationMyD88 (S34Y)PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRTagsExpressionYeastMutationChanged sequence to remove a MluI cut sitePromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
cTRE-LSL-GFP-IRES-Cas9-sgCR8
Plasmid#135668PurposeIntroduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertsgCR8
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB
Plasmid#131350PurposeExpresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase BDepositorInsertMyD88-GyrB (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-IRES-EGFP
Plasmid#153949PurposepiggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFPDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsFLAGExpressionMammalianMutationT910MPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32474PurposeExpresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP markerDepositorInsertPSAMQ79G,Q139G-5HT3 HC
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAG-FNLS-T2A-EGFP-ires-puro
Plasmid#121169PurposeCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.DepositorInsertFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro
Plasmid#110831PurposeExpresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.DepositorInsertAzgp1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsdTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
M10.2-IRES-tauVenus-ACNF Targeting Vector
Plasmid#15858DepositorInsertM10.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry
Plasmid#75133PurposeRetroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49229PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mouse pGL3-ank3_1-promoter firefly luciferase
Plasmid#86436PurposeMouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1eDepositorInsertank3_1 promoter
UseTagsn/aExpressionMammalianMutationn/aPromoterankyrin-3_1Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only