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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC6-GPT2-3xFLAG

Plasmid

#163449

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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DsRed2_pLENTI-CAG-IRES-GFP

Plasmid

#177010

Purpose

Mammalian lentiviral expression vector encoding DsRed2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgSTAT3-1

Plasmid

#121425

Purpose

sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgSTAT3-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-GK in PLJM60

Plasmid

#154207

Purpose

Lentiviral vector for stable expression of GK

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GK (GK Human)

Use

Lentiviral

Tags

N terminal FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, F124D

Plasmid

#180648

Purpose

Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, F124D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4

Plasmid

#110158

Purpose

Lentiviral vector for expression of human TFAP4 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCSE1L v1 puro

Plasmid

#192345

Purpose

Lentiviral expression vector for an inducible shCSE1L v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCSE1L v1 (CSE1L Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCSE1L v2 puro

Plasmid

#192346

Purpose

Lentiviral expression vector for an inducible shCSE1L v2)

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCSE1L v2 (CSE1L Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT2-3xFLAG

Plasmid

#163447

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-DoxOn-shCUX1-5150

Plasmid

#90469

Purpose

Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #2

Plasmid

#83090

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170816

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170817

Purpose

Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUCHR-CD5Flag2-P2A-smAID-mClover

Plasmid

#110657

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5Flag2-P2A-AID-mClover

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMA3431

Plasmid

#46876

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

R592Q

Promoter

TRE-Tight

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #1

Plasmid

#83088

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only