We narrowed to 6,523 results for: sas;

1. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPXL-Klf4

   Plasmid

   #36981

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pBS-f1(-)-ACTB-mEGFP

   Plasmid

   #115917

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP
   

   Use

   Donor template

   Tags

   EGFP

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos3.2FP

   Plasmid

   #203757

   Purpose

   Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   NRas c-term (NRAS Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a-SARS-CoV2-NP-Nt(47-173)

   Plasmid

   #165091

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBJG1 T7-8xHis-OGT D554N

   Plasmid

   #154279

   Purpose

   Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   Aspartate 554 changed to Asparagine- bacterial co…

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1 FLCN (R164A) FLAG GFP

   Plasmid

   #164980

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   R164A

   Promoter

   UBC

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas4_1_2_VA

   Plasmid

   #186438

   Purpose

   Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCas9 PE2*-3HA

    Plasmid

    #169854

    Purpose

    Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-star-M-MLV-3HA
    

    Tags

    3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-f1(-)-GAPDH-IRES-EGFP

    Plasmid

    #115918

    Purpose

    donor to insert IRES-EGFP at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-EGFP
    

    Use

    Donor template

    Tags

    dTomato

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LUC2 (L/Sec/deltaP)

    Plasmid

    #112145

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and d…

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-f1(-)-TOMM20-mEGFP

    Plasmid

    #115923

    Purpose

    donor to insert EGFP at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-mEGFP
    

    Use

    Donor template

    Tags

    EGFP

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO mouse shRNA 1 DEPTOR

    Plasmid

    #21337

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 1 (Deptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pSCT-GAL93-NONO

    Plasmid

    #46325

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    NONO (NONO Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET32a-HD39Q

    Plasmid

    #11514

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 39 glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 39 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET32a-Spike-S-4P

    Plasmid

    #165094

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    March 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBABE-LynG

    Plasmid

    #206867

    Purpose

    Expression of LynG probe - LynAnchor-eGFP

    Depositor

    Dirk-Peter Herten

    Article

    Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

    Insert

    LynG
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains Lyn amino acids 1-16

    Available Since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1 FLCN (F118D) FLAG GFP

    Plasmid

    #164979

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    F118D

    Promoter

    UBC

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBoBi-hALDOA

    Plasmid

    #210806

    Purpose

    For stably expression in cells

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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