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pAAV-CMVenh synapsin-intron-mCherry-1x miR30 SCR

Plasmid

#216393

Purpose

transfer plasmid for AAV vector production of mCherry and miR scramble (no target)

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR control

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 VPS13C-TSS-guide1

Plasmid

#125476

Purpose

CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBV163

Plasmid

#108364

Purpose

Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

Depositor

Scott Moye-Rowley

Article

Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

Insert

Knock-in Cg TDH3 promoter in Cg YAP1 gene

Use

Replace c.glabrata yap1 promoter with c. glabrata…

Promoter

TDH3

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.28.3z

Plasmid

#200678

Purpose

Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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PGK-IKZF3-CTERM-GFP

Plasmid

#185779

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Beta1-N-18

Plasmid

#55064

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_gRNA_2

Plasmid

#196626

Purpose

Knock-out STING in murine cells, gRNA 2.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 2 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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