We narrowed to 5,258 results for: pcdna3.1

1. CENP-B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45214

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Stra6L-6His

   Plasmid

   #174215

   Purpose

   Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

   Depositor

   Adrian Press

   Article

   Stafast et al ACS Appl. Polym. Mater. 2022

   Insert

   Stra6L (Stra6l Mouse)
   

   Use

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   non

   Promoter

   CMV

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. WNK2 K207A

   Plasmid

   #24570

   Depositor

   Joseph Costello

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

   Insert

   WNK2` (WNK2 Human)
   

   Use

   Tags

   His6 and V5

   Expression

   Mammalian

   Mutation

   K207A

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGAL4-DBD-MCS-long

   Plasmid

   #145246

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-ZnF/HIT1

   Plasmid

   #15332

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   zinc finger, HIT domain containing 1 (ZNHIT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. POLArIS_NbVim-cp10GFP

   Plasmid

   #171020

   Purpose

   Expresses NbVim-cp10GFP#2 in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

   Insert

   anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
   

   Use

   Tags

   cp10GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP Rab4

   Plasmid

   #99018

   Purpose

   Localization and visualization of trafficking protein Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 (Rab4 Synthetic, Fly)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFP Rab4DN

   Plasmid

   #99019

   Purpose

   Localization and visualization of the dominant negative form of Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 DN (Rab4 Synthetic, Fly)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   S22N Dominant negative

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid

    #188992

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K109R, P245A, G328S, G381V, G429D

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid

    #114187

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and mEOS2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 RL3Sc

    Plasmid

    #109365

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HCMVgL-FLAG (Merlin)

    Plasmid

    #136450

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

16. Dr-EGFR

    Plasmid

    #184004

    Purpose

    Dr-EGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-EGFR (EGFR Human)
    

    Use

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 PBD wt (Nigg AH12)

    Plasmid

    #41162

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603)

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pER-eZinCh-2

    Plasmid

    #70009

    Purpose

    expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    ER-eZinCh-2
    

    Use

    Tags

    KDEL sequence and PPI signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmyc-GFP-TNRC6A-NES-mut

    Plasmid

    #42001

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-YL1

    Plasmid

    #15333

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

    Insert

    transcription factor-like 1 (VPS72 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

25. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA_DiCre_60.F2

    Plasmid

    #60208

    Purpose

    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
    

    Use

    Tags

    FRAP and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 2021-2112 of FRAP with a T2098L mutat…

    Promoter

    CMV

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Bongwoori

    Plasmid

    #63720

    Purpose

    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Bongwoori
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA-SCW-HHL

    Plasmid

    #190634

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169591

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-HA-CAML

    Plasmid

    #22284

    Depositor

    Erik Lillehoj

    Article

    Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

    Insert

    calcium-modulating ligand (CAMLG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

38. b2-TS3

    Plasmid

    #103877

    Purpose

    full-length human b2 with tension sensor at b2_733

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

40. aL-mEos3.2

    Plasmid

    #113154

    Purpose

    constrained LFA-1-mEos3.2 fusion

    Depositor

    Timothy Springer

    Article

    Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

    Insert

    full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
    

    Use

    Tags

    mEOS3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge