We narrowed to 4,495 results for: Ado

1. EF1a_HNF1A_P2A_Hygro_Barcode

   Plasmid

   #120448

   Purpose

   Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HNF1A (HNF1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_KLF6_P2A_Hygro_Barcode

   Plasmid

   #120492

   Purpose

   Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_RUNX1_P2A_Hygro_Barcode

   Plasmid

   #120475

   Purpose

   Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

   Plasmid

   #120509

   Purpose

   Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaLZ (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of leucine zipper motif

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

   Plasmid

   #120508

   Purpose

   Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaHLH (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of helix-loop-helix motif

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro-2xMyc-Rac1-WT

   Plasmid

   #128580

   Purpose

   For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CamKII-ADAM23-pHluorin

   Plasmid

   #185539

   Purpose

   Expresses ADAM23-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   ADAM23 (Adam23 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHDM-SARS-CoV-2 Δ18

    Plasmid

    #164434

    Purpose

    Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-xBE4Gam-P2A-Puro

    Plasmid

    #112676

    Purpose

    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE4Gam
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX MLKL(wt)--2A-mCherry-puro

    Plasmid

    #231974

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_KLF4_P2A_Hygro_Barcode

    Plasmid

    #120455

    Purpose

    Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. optoFcR

    Plasmid

    #224291

    Purpose

    Expression of an optogenetic Fc Receptor

    Depositor

    Meghan Morrissey

    Article

    Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

    Insert

    Fc receptor gamma (Fcer1g Mouse)
    

    Use

    Lentiviral

    Tags

    mScarlet-Cry2-olig and myristoylation sequence

    Expression

    Mutation

    aa 45-86 only

    Promoter

    SFFV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX_hDDX5_FL_D248N

    Plasmid

    #88872

    Purpose

    inducible expression of GST-DDX5 D248N in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D248N

    Promoter

    Tac

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid

    #120513

    Purpose

    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Thr58Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Threonine to Alanine at a…

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF1-WT_RASA2-EF1-Puro

    Plasmid

    #114096

    Purpose

    Express RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEZYeGFP-SARS-CoV-2E GGG

    Plasmid

    #224582

    Purpose

    To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

    Insert

    SARS-CoV-2E GGG (E SARS-CoV-2)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PBM (LLV) mutated to GGG

    Promoter

    CMV

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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