We narrowed to 3,022 results for: ER
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Plasmid#167353PurposeddPCR gating control for droplets containing 5'pol or 3'pol targetsDepositorInsertpol
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_17_005
Plasmid#167351PurposeddPCR gating control for droplets containing gat and tat targetsDepositorInsertgat_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron-ST
Plasmid#119034PurposeExpress soma-localized Voltron in mammalian cellsDepositorInsertVoltron-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-G610C
Plasmid#119827PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorUseTagsGST and HisExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gEgIL
Plasmid#11622DepositorInsertHSV-1 glycoprotein E and HSV-1 glycoprotein I
UseTagsHisExpressionInsectMutationDicistronic vector containing HSV-1 gE (KOS strai…PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184V
Plasmid#106175PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterhSynapsinAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-STAT3mts
Plasmid#195577PurposeExpresses STAT3 with mitochondrial targeting sequenceDepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsCox8 presequenceExpressionMammalianMutationPromoterCMV enhancer and promoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG 5E
Plasmid#185341PurposeAssessing G2BR function in AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFlag, 6xHisExpressionMammalianMutationR382E/Q383E/K390E/R398E/R400EPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
Plasmid#217676PurposeAAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.DepositorInsertGtACR2-P2A-Voltron2ST
UseAAVTagsSoma targeting sequence (Kv2.1) on Voltron2 gene …ExpressionMammalianMutationPromoterEF‐1αAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
UseTagsExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG-DCUE
Plasmid#185339PurposeAssessing domain requirements of AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFLAG, 6xHisExpressionMammalianMutationdeletion of aa 293-337 corresponding to the CUE d…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-2X-P2A-Puro
Plasmid#110840PurposeLentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)DepositorInsertBE3RA-2X
UseLentiviralTagsExpressionMutationD10A and 2 tandem NLS sequences in the XTEN linkerPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NES-NIR-GECO1
Plasmid#113683PurposeExpress NIR-GECO1 preferentially in neuronsDepositorInsertNES-NIR-GECO1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα1(I)
Plasmid#119842PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT
Plasmid#134645Purposelysosome-targeted Sac1 catalytic domain wild typeDepositorInsertSac1 catalytic domain (77-520 aa) (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationcontains only amino acids 77-520 (Please see depo…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xFNLS-P2A-Puro
Plasmid#110872PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS(3.7)
UseLentiviralTags3X FLAGExpressionMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…PromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only