We narrowed to 13,968 results for: SHI

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pGEX6p-1-UFBP1 M4

Plasmid

#204562

Purpose

Expresses GST-tagged UFBP1 M4 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

116-214aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 F196A V198A

Plasmid

#204564

Purpose

Expresses GST-tagged UFBP1 F196A V198A in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

F196A V198A

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3 K214R

Plasmid

#204570

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

K214R

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3

Plasmid

#204569

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

none

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-GABARAP

Plasmid

#204574

Purpose

Expresses Ash-tagged GABARAP in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Mutation

deltaL117

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-oScarlet-WPRE

Plasmid

#183520

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mDrc3-PA

Plasmid

#176467

Purpose

Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

dynein regulatory complex subunit 3 (Drc3 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183529

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

Ef1a

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-ChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183525

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mAC-POLR2A donor (Hygro)

Plasmid

#124496

Purpose

A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)

Use

CRISPR

Tags

mAID-mClover

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-hsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183527

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

H33R

Promoter

Ef1a

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-2A-SOX17-GR-IH

Plasmid

#165079

Purpose

Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Inserts

rtTA-M2
SOX17 (SOX17 Human)

Use

Piggybac transposition

Tags

Glucocorticoid Receptor

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-B

Plasmid

#67523

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

Depositor

Kazuto Kobayashi

Article

Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

Insert

Fusion Glycoprotein type B

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3(+)-UFBP1 F196A V198A-MYC

Plasmid

#204556

Purpose

Expresses FLAG-tagged UFBP1 F196A V198A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Mammalian

Mutation

F196A V198A

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-LC3B

Plasmid

#204573

Purpose

Expresses Ash-tagged LC3B in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

LC3B (MAP1LC3B Human)

Expression

Mammalian

Mutation

delta121-125aa

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-3xFLAG-CMV14-CYB5R3 K214R

Plasmid

#204552

Purpose

Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

K214R

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETCON-IgG1/design_50

Plasmid

#30301

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG1

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB20/design_70

Plasmid

#30254

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB20

Expression

Yeast

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB28/design_25

Plasmid

#30260

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB28

Expression

Yeast

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only