We narrowed to 32,645 results for: uros

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pBABE-puro SHP2 D61A

Plasmid

#8330

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D61A (activated mutant)

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-xBE3-P2A-Puro

Plasmid

#110870

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE3

Use

Lentiviral

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 Cherry Puro

Plasmid

#64340

Purpose

Bicistronic expression of Centrin-mCherry fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Tags

IRES-Puro and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-O-KLF7-T2A-PuroR

Plasmid

#162346

Purpose

Lentiviral expression of KLF7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

KLF7 (KLF7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-PARG shRNA

Plasmid

#23260

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

poly(ADP-ribose) glycohydrolase shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro dn-cHSF1

Plasmid

#134728

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Mouse, Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Puro-shPP2A-Abeta

Plasmid

#15250

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-IFITM3g1 (BB15)

Plasmid

#139460

Purpose

Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E4-T2A-Puro

Plasmid

#190810

Purpose

Express APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Mutation

E4

Promoter

Ubi C

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only