159,557 results

1. Flag-HA-GFP

   Plasmid

   #22612

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. LXRalpha_pcDNA3.1

   Plasmid

   #177621

   Purpose

   Full-length human LXR alpha cDNA sequence in pcDNA3.1+

   Depositor

   Thomas Burris

   Article

   Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

   Insert

   LXR alpha (NR1H3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EGFP-P4M-SidMx2

   Plasmid

   #136997

   Purpose

   Lentiviral EGFP lipid sensor for PI(4)P

   Depositor

   Ken-Ichi Takemaru

   Insert

   EGFP-P4M-SidMx2
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_22A

   Plasmid

   #91133

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAM18dRGA[MCS]

   Plasmid

   #84683

   Purpose

   Can be used for the transformation of rickettsia

   Depositor

   Ulrike Munderloh

   Article

   Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-acta2

   Plasmid

   #78686

   Purpose

   acta2 promoter for Gateway cloning

   Depositor

   Sarah Childs

   Article

   Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

   Insert

   acta2 promoter (acta2 Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   acta2

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2536

   Plasmid

   #142397

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX6 (SOX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. yeast_DualReporter

   Plasmid

   #127546

   Purpose

   A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

   Depositor

   Aviv Regev

   Article

   de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

   Inserts

   bla
   mCherry
   URA3 (URA3 Budding Yeast)
   yEVenus
   Nat1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmScarlet3-alphaTubulin_C1

    Plasmid

    #189768

    Purpose

    Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-alphaTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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