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pTC3RBSC1cI

Plasmid

#193773

Purpose

Expresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX*C3E2-Crimson

Plasmid

#193754

Purpose

Expresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLUX*

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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PRC3MKO2

Plasmid

#193757

Purpose

Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mKO2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PR

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLC3RBSC2cI

Plasmid

#193775

Purpose

Expresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLLacO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmGW182-RBD_F

Plasmid

#146290

Purpose

Bacterial Expression of DmGW182-RBD

Depositor

Elisa Izaurralde

Article

Eulalio et al Nucleic Acids Res. 2009 Mar 18. ():.

Insert

DmGW182-RBD (gw Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-delRBD_E

Plasmid

#146229

Purpose

Insect Expression of DmGW182-delRBD

Depositor

Elisa Izaurralde

Article

Eulalio et al Nucleic Acids Res. 2009 Mar 18. ():.

Insert

DmGW182-delRBD (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-767del664-673_J

Plasmid

#146671

Purpose

Bacterial Expression of HsPatL1_517-767-del664-673

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-767-del664-673 (PATL1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_70-770del399-516_J

Plasmid

#146682

Purpose

Mammalian Expression of HsPatL1_70-770del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770del399-516 (PATL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1-del399-516_J

Plasmid

#146686

Purpose

Mammalian Expression of HsPatL1-del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-del399-516 (PATL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_500-968-dsRNAres_J

Plasmid

#146693

Purpose

Insect Expression of DmHPat_500-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_500-968-dsRNAres (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_500-684_H

Plasmid

#146470

Purpose

Insect Expression of DmHPat_500-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_500-684 (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_685-968_H

Plasmid

#146471

Purpose

Insect Expression of DmHPat_685-968

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_685-968 (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat-del57-499_H

Plasmid

#146472

Purpose

Insect Expression of DmHPat-del57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499 (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS myr-c-P3k

Plasmid

#196373

Purpose

Oncogenic with addition of a myristylation signal and expresses in avian cells

Depositor

Peter Vogt

Article

Chang et al Science. 1997 Jun 20;276(5320):1848-50.

Insert

Myr-c-P3k-FLAG

Use

Retroviral; Avian

Tags

flag and myristoylation

Expression

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag-16S rRNA Left_G1397C-ABE

Plasmid

#189642

Purpose

16S rRNA Left_G1397C-ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0120

Plasmid

#172791

Purpose

Level 0 module containing CDS2ns_mHSP21

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature HSP21 (44-227), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0180

Plasmid

#172814

Purpose

Level 1 module containing TP-CFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-CFP-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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