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pGEX Grb10-SH2

Plasmid

#46437

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb10 (GRB10 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 435-548

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp2-SH2

Plasmid

#46460

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp2 (BCAR3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 144-259

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin2-SH2

Plasmid

#46416

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin2 (CHN2 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 49-155

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 35s:PIF6:tNos (GB0170)

Plasmid

#160606

Purpose

TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

PIF6

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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KA2944_pPB-TREtight-Esrrb-FLAG-InO

Plasmid

#124179

Purpose

piggyBac-based Dox-inducible expression of Esrrb-FLAG

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Arg-SH2

Plasmid

#46405

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Arg/Abl2 (ABL2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 151-257

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Dapp1-SH2

Plasmid

#46422

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Dapp1 (DAPP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 25-151

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR C10S S85C F87E

Plasmid

#238074

Purpose

Bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S S85C F87E

Promoter

T7

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-193

Plasmid

#197137

Purpose

CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

mCherry(M1V/M9A)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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