Addgene

We narrowed to 6,038 results for: WUS;

Showing: 2851 - 2880 of 6038 results

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

cdk-1

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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hCEP120-Myc

Plasmid

#50381

Purpose

Tet-On inducible expression of Myc-tagged hCEP120

Depositor

Tang Tang

Article

Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

Insert

Human CEP120 (CEP120 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

This hCEP120 contains 4 SNPs (rs6876883, rs659544…

Promoter

CMV-2xTetO

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-UMI

Plasmid

#222694

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Promoter

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pXZX501

Plasmid

#169455

Purpose

pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATa

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Use

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Promoter

Available since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Trak1

Plasmid

#127621

Purpose

N-terminal GFP-tagged mouse Trak1

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Trak1 (Trak1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ-dpcoCas9-Act3.0

Plasmid

#158408

Purpose

CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK3

Plasmid

#172993

Purpose

constitutive expression of FLAG-tagged TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK3 (TAOK3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pXZX351

Plasmid

#169454

Purpose

pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATα

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-PGC1(1-400)

Plasmid

#1029

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-400

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P202A/F203A)-FLAG

Plasmid

#164976

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P202A/ F203A

Promoter

EF1a

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

Plasmid

#197952

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26 and pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-His RTN3A1

Plasmid

#31087

Depositor

Jimtong Horng

Article

Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

Insert

reticulon 3 (RTN3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-MtAcKRS (human-opti)

Plasmid

#164195

Purpose

To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtAcKRS-478(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

D76G; L325M; L329I; Y330F; L333A; C372F

Promoter

CMV

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIID-K43M

Plasmid

#186830

Purpose

Expresses FLAG-CaMKIID-K43M in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2d (Camk2d Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 43 to Methionine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgMyc_1

Plasmid

#138324

Purpose

Express guide RNA 2 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGIG_V5-PPP6C

Plasmid

#92013

Purpose

Expresses V5-PPP6C, includes IRES-Emerald

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

PPP6C (PPP6C Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF2_P2A_Hygro_Barcode

Plasmid

#120489

Purpose

Barcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF2 (KLF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MTK0_003

Plasmid

#123925

Purpose

Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Cas9-sgRNA destination

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3C-BE3

Plasmid

#113412

Purpose

Expresses hA3C-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3C-BE3 (APOBEC3C Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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