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pCITF-KCC2

Plasmid

#61404

Purpose

Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

Depositor

Franck Polleux

Article

Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

Insert

KCC2 (SLC12A5 Human)

Use

Tags

IRES-tdTomato-Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-N

Plasmid

#64318

Purpose

GFP-tagged mammalian expression of N-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-mRuby3

Plasmid

#171154

Purpose

Encodes mRuby3 fluorophore

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TCB

Plasmid

#48332

Purpose

Cre-dependant TVA-mCherry fusion

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry

Use

AAV

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSLS.400

Plasmid

#187201

Purpose

Expresses retron Eco4 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco4 Reverse Transcriptase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

mphR

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-dsRed

Plasmid

#73460

Purpose

Expresses dsRed for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

dsRed

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry

Plasmid

#73464

Purpose

Expresses mCherry for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS/ES-EnvA-VSVg-WPRE-pA

Plasmid

#87221

Purpose

to package wild type EnvA psudotyped lentivirus

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

EnvA-VSVg

Use

Mouse Targeting and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-Cre

Plasmid

#73474

Purpose

Expresses Cre for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

Cre recombinase

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-I-N-CAAX

Plasmid

#41640

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-IntersectinDH-Dronpa145N-CAAX

Use

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-FlpO

Plasmid

#73473

Purpose

Expresses FlpO for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Use

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
RabV CVS-N2c(deltaG)-GCaMP6f

Plasmid

#73466

Purpose

Expresses GCaMP6f for calcium imaging

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

GCaMP6f

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS N2c(deltaG)-synaptophysin-GFP

Plasmid

#73492

Purpose

Expresses EGFP for monosynaptic circuit mapping at the synapse

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

synaptophysin-GFP

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-NRSE-RFP

Plasmid

#22929

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pACCS3-1

Plasmid

#78559

Purpose

4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.

Depositor

James Kaper

Article

Giron et al Vaccine. 1995 Jul;13(10):939-46.

Insert

cs3 operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

tet

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Gucy1b2-B

Plasmid

#105457

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-A

Plasmid

#105456

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKEK3225

Plasmid

#234087

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

DasherGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23100-BsaI

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23100-BsaI

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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