We narrowed to 7,393 results for: Trac

1. pALS3-Ma-SUMO-TAG35-sfGFP

   Plasmid

   #212120

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-TAG35-sfGFP
   M. alvus Pyl-tRNA
   

   Use

   Tags

   His6 and SUMO-TAG35

   Expression

   Bacterial

   Mutation

   E35TAG (within SUMO encoding sequence)

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO, native, native (respectively)

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTYF-sGFAP-IRES-tdTomato

   Plasmid

   #166920

   Purpose

   Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   sGFAP

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVCERC-5 Pvan-dcas9 (Sth3)

   Plasmid

   #133319

   Purpose

   expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   vanillate

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAN147

   Plasmid

   #129202

   Purpose

   yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSV(X) C/EBPdelta

   Plasmid

   #13394

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPdelta (Cebpd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEx-insertion-tet

   Plasmid

   #172211

   Purpose

   For genetic insertion in tetracyclin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Genetic insertion in prevotella copri

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEH280 NLS dTomato 3x high affinity MS2 loops

   Plasmid

   #168211

   Purpose

   Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   NLS dTomato
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-EcPPK1

   Plasmid

   #159279

   Purpose

   Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

   Insert

   HA-tagged Polyphosphate Kinase 1
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CFP-FRBx5

    Plasmid

    #103774

    Purpose

    expresses CFP-FRBx5 protein in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    CFP-FRBx5
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CamK2-tTA

    Plasmid

    #210731

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CamK2

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPI278

    Plasmid

    #113227

    Purpose

    extrachromosomal expression vector, expression of H2B-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    H2B
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    act15

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113344

    Purpose

    Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAL119

    Plasmid

    #21909

    Depositor

    Maria Castro

    Article

    Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

    Insert

    hCMV promoter expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113342

    Purpose

    Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV LDTS-LIRmut

    Plasmid

    #189001

    Purpose

    lipophagy-inducing adaptor protein, negative control

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-LIRmut
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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