We narrowed to 3,248 results for: cat.3

1. pBP-J23108

   Plasmid

   #72964

   Purpose

   Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23108 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBP-J23114

   Plasmid

   #72965

   Purpose

   Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23114 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-T7_RBS

   Plasmid

   #72978

   Purpose

   T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-Strep(II)

   Plasmid

   #72984

   Purpose

   Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Strep(II) N-terminal tag
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTU2-b-RFP

   Plasmid

   #72959

   Purpose

   Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - b site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

6. double_sgRNA targeting e3 and e7

   Plasmid

   #190689

   Purpose

   sgRNAs targeting enhancer 3 and 7 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 3 and 7 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-Pdha1

   Plasmid

   #175936

   Purpose

   knockout mouse Pdha1

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Pdha1 gRNA (Pdha1 Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIAeBlue

   Plasmid

   #185891

   Purpose

   In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIEforRed

   Plasmid

   #185892

   Purpose

   In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6_rd12_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232435

    Purpose

    pegRNA with optimized 3' modifications to correct the rd12 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

    Plasmid

    #194248

    Purpose

    Expresses 3 miRNAs targeting mouse alpha-Synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRaSynuclein(mouse) (Snca Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHnS KI;Gria1 Donor;3xV5 KO;Dlg4

    Plasmid

    #240294

    Purpose

    KI:Gria1 Donor:3xV5 KO:Dlg4

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Gria1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHnS KI;Lmnb1 Donor;3xV5 KO;Rbfox3

    Plasmid

    #240299

    Purpose

    KI:Lmnb1 Donor:3xV5 KO:NeuN

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Lmnnb1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHnS KI;Sptbn4 Donor;3xV5 KO;Nfasc

    Plasmid

    #240296

    Purpose

    KI:Sptbn4 Donor:3xV5 KO:Nfasc

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Sptbn4
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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