163,606 results

1. pTIPI_mK_Anti-HA [12CA5]

   Plasmid

   #210657

   Purpose

   Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Anti-HA [12CA5] kappa light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Mouse kappa light chain

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pb-CAGGS-dCas9-blasto

   Plasmid

   #218555

   Purpose

   Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

   Depositor

   Alejandro Chavez

   Insert

   dCas9
   

   Use

   CRISPR; Piggybac transposition

   Tags

   2xSV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hNeuroD1

   Plasmid

   #233184

   Purpose

   Retroviral expression of NeuroD1

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   NeuroD1 (NEUROD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-mNgn2

   Plasmid

   #233148

   Purpose

   Retroviral expression of Ngn2

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubi:3xHA-ccdb + RUBY

   Plasmid

   #233307

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker

   Depositor

   Thomas Baum

   Insert

   Ubi:3xHA-ccdb + RUBY
   

   Use

   Gateway destination

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA_AT

   Plasmid

   #196243

   Purpose

   Backbone plasmid for cloning sgRNA

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SNAP-CD86iso1

   Plasmid

   #223601

   Purpose

   Lentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 isotype1 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPI4-Strep-SNAP-CD28-GCN4-His6

   Plasmid

   #223585

   Purpose

   Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMVV052

   Plasmid

   #215303

   Purpose

   Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PRhaB

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

    Plasmid

    #216162

    Purpose

    Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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