We narrowed to 2,190 results for: D10

1. E1b-GFP-Tol2-Gateway dre SE-irf2bpl B

   Plasmid

   #89371

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

   Plasmid

   #89373

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

   Plasmid

   #89370

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139093

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dxCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-CBE4max-SpRYc-P2A-EGFP

   Plasmid

   #208336

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CAG

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156938

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA4A (SEMA4A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FRIG-myc-Sema4D-CD4

    Plasmid

    #51606

    Purpose

    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4D/CD4-FLAG (Sema4d Human, Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 660-861 were replaced with CD4…

    Promoter

    RSV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-xF2X-P2A-Puro

    Plasmid

    #110873

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS-2X(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid

    #129026

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis

    Plasmid

    #157486

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA7A (SEMA7A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CYLBL_Neo_CAGdCas9-KRAB

    Plasmid

    #220439

    Purpose

    CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

    Depositor

    Yin Shen

    Article

    Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

    Insert

    dCas9
    

    Use

    CRISPR and TALEN

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge