We narrowed to 8,586 results for: Ott

1. pRSF-G1(CysCNP)RS

   Plasmid

   #225054

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Chem 102385

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNMTMA_MBP-Z-Cav1-His6

   Plasmid

   #223701

   Purpose

   To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

   Depositor

   Rob Parton

   Article

   T. D. et al bioRxiv

   Insert

   MBP-Z-Cav1-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNoT7

   Plasmid

   #218168

   Purpose

   pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

   Depositor

   Emily Weinert

   Article

   Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

   Insert

   Short sequence, mutated RBS and T7 sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA - mutated T7

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJA008

   Plasmid

   #128512

   Purpose

   Expresses Xylanase C

   Depositor

   Marcos Alcocer

   Article

   Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

   Insert

   Xylanase C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Histone 4B

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW228

   Plasmid

   #113242

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   TTGGTAG->tAggtag

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW227

   Plasmid

   #113241

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   TTGGTAG->Atggtag

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAW207

   Plasmid

   #113247

   Purpose

   E. coli expression vector for IHFa K20A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa K20A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K20A

   Promoter

   T7

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW205

   Plasmid

   #113245

   Purpose

   E. coli expression vector for IHFa D14A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa D14A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D14A

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW217

   Plasmid

   #113239

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GGTAG->ggtaC

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW211

    Plasmid

    #113233

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->AACGA

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW210

    Plasmid

    #113189

    Purpose

    E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H208A

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW203

    Plasmid

    #113243

    Purpose

    E. coli expression vector for IHFa E10A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa E10A (ihfA E. coli)
    IHFb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E10A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBSV2G_PflaB-msfGFPBb

    Plasmid

    #118231

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven msfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiSAMPHv2

    Plasmid

    #167934

    Purpose

    All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. psiCHECK-2-XNS

    Plasmid

    #196655

    Purpose

    Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lyn-tailed mCherry-SEpHluorin

    Plasmid

    #32002

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    Membrane-targeting sequence of Lyn kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmScarlet_C1

    Plasmid

    #85042

    Purpose

    Mammalian expression of mScarlet

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLifeAct_mScarlet_N1

    Plasmid

    #85054

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBSV2G_PflaB-iRFPBb

    Plasmid

    #118237

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven iRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge