We narrowed to 9,280 results for: Pol
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Plasmid#140860PurposeSame as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)DepositorInsertCbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
ExpressionBacterialMutationdeleted amino acids 1-235 of CsoS2Available SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz76_CB_operon_CsoS2_N4
Plasmid#140858PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
ExpressionBacterialMutationdeleted amino acids 1-189 of CsoS2Available SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg1
Plasmid#113966PurposeSingle short guide RNA targeting GTATAGCATACATTATACGDepositorInsertsg1
PromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM136
Plasmid#134177PurposeBacterial expression of his-CENP-C 700-943DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-OEP7(1–50)
Plasmid#100601PurposeGateway entry vector for AtOEP7(1–50)DepositorInsertOEP7(1–50) (OEP7 Mustard Weed)
UseGateway entry vectorAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA-dabAB2
Plasmid#133012PurposepFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)DepositorInsertsdabB2
dabA2
ExpressionBacterialPromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2 C351A
Plasmid#133005PurposepFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialMutationC351APromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
YCe1885 HC_Kan_Kozak-ATG-MLS_p6
Plasmid#100676Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak-ATG-MLS
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe2735 HC_Kan_Kozak-ATG-IgkL_p6
Plasmid#100697Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak_ATG_IgkL
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
CTL2 (M2225R)
Plasmid#96952PurposeExpresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coliDepositorInsertCTL2 (M222R) of PIEZO1
Tags6x Histidine TagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - MPX
Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-1-CTS
Plasmid#86067PurposeA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-1 (PKD1 Human)
TagsGSTExpressionBacterialMutationcontains the region from 4297-4303PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TDR
Plasmid#89627PurposeExpresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG inductionDepositorInsertthiol dependent reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23108
Plasmid#72964PurposeLevel 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23108 promoter
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23114
Plasmid#72965PurposeLevel 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23114 promoter
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS
Plasmid#72978PurposeT7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-lacZ
Plasmid#72950PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-B-lacZ
Plasmid#72951PurposeLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - B site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-C-lacZ
Plasmid#72952PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-lacZ
Plasmid#72953PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-B-RFP
Plasmid#72955PurposeLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - B site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-C-RFP
Plasmid#72956PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-RFP
Plasmid#72957PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b-RFP
Plasmid#72959PurposeLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABCDepositorInsertLevel 2 - b site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA2
Plasmid#73984PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA1
Plasmid#73985PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA2
Plasmid#73986PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA2
Plasmid#73980PurposeOtx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ZFYVE9
Plasmid#67134PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCGS652
Plasmid#62220PurposeMammalian expression TALEN vector with N152, C+18 truncationsDepositorTypeEmpty backboneUseTALENTagsAcV5 and SV40 NLSExpressionMammalianPromoterminiCaggsAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myc-CICf_AEA
Plasmid#48188PurposeExpresses AEA mutated CicDepositorAvailable SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60B
Plasmid#48740PurposeBacterial expression of UNC-60B (a C. elegans ADF/cofilin)DepositorAvailable SinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60A
Plasmid#48739PurposeBacterial expression of UNC-60A (a C. elegans ADF/cofilin)DepositorAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Phb
Plasmid#48162Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Phb (Phb Mouse)
UseLuciferaseAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15 mod
Plasmid#48161Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mrpl1
Plasmid#48166Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of MRPL11 (Mrpl11 Mouse)
UseLuciferaseAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15
Plasmid#48160Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
Syntaxin-4 M2 (Y115A, Y148A, Y251A)
Plasmid#44030DepositorInsertSyntaxin-4
Tags2xMyc and HisExpressionMammalianMutationY115A Y148A Y251AAvailable SinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFM113
Plasmid#40159DepositorTagsGSTExpressionBacterialMutationN terminal portion of protein (aa1-364)PromotertacAvailable SinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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