We narrowed to 8,313 results for: reporter

1. CAG-Cas9

   Plasmid

   #89995

   Purpose

   Expresses high levels of WT Cas9 for genome editing

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   hCas9
   

   Tags

   3xFlag, SV40 NLS, and T2A peptide

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207181

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Promoter

   SFFVp

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FlagETR3 (CELF2)

   Plasmid

   #96900

   Purpose

   expression of Flag-tagged human CELF2 (aka CUGBP2)

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human CELF2 cDNA (CELF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   No promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMPRAv3:minP-GFP

   Plasmid

   #109036

   Purpose

   MPRA v3 minimal promoter with GFP donor

   Depositor

   Pardis Sabeti, Ryan Tewhey

   Article

   Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

   Insert

   emGFP
   

   Expression

   Mammalian

   Promoter

   minP

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. XLone-SOX17

   Plasmid

   #135764

   Purpose

   expresses human SOX17 upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   SOX17 (SOX17 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   TRE3G

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TB:6xAP-1_RE:MiniP

   Plasmid

   #133884

   Purpose

   6 copies of the AP-1 binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   6 copies of the AP-1 binding motif
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. dClover2-C1

    Plasmid

    #54577

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

    Depositor

    Michael Davidson

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Type

    Empty backbone

    Tags

    dClover2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti-mU6-Luc2GO-PGK-Neo

    Plasmid

    #136905

    Purpose

    Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    Luc2CyGo
    

    Use

    Lentiviral

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hRARγ.hRXRα

    Plasmid

    #135416

    Purpose

    Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-gamma
    Human retinoid X receptor-alpha
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. P2A-mCherry-EhT backbone

    Plasmid

    #215549

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ShuttleNSE

    Plasmid

    #50958

    Purpose

    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    NSE

    Available Since

    Feb. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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