We narrowed to 53,290 results for: SON

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pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

Plasmid

#85716

Purpose

Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Nme dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO and U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRM055 His-TEV-Cas9 6xNLS 0C

Plasmid

#196246

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Mito-7

Plasmid

#55433

Purpose

Localization: Mitochondria, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Mito (COX8A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mIFP-H2B-C-10

Plasmid

#56224

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

Plasmid

#113436

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

Plasmid

#117405

Purpose

SpyCas9 sgRNA 1 targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SpyCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

Plasmid

#136514

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

Depositor

Erik Sontheimer

Article

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

Codon-optimized AcrIIA5

Use

Lentiviral

Tags

FLAG/NLS

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Caveolin-C-10

Plasmid

#55404

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Caveolin (CAV1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Golgi-7

Plasmid

#55420

Purpose

Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Golgi (B4GALT1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
HES7-NLuc-2A-tdTomato

Plasmid

#130932

Purpose

Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Tags

NanoLuc and tdTomato

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

Plasmid

#117406

Purpose

SpyCas9 sgRNA 2 targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SpyCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-LaminB1-10

Plasmid

#55425

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
PUC57_BAG3P209L-eGFP

Plasmid

#98183

Purpose

Codes for full length, GFP tagged mutant Bag3

Depositor

Robert Bryson-Richardson

Article

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3P209L-eGFP (BAG3 Human)

Tags

GFP

Expression

Bacterial

Mutation

P209L

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-6

Plasmid

#56223

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-Fibrillarin-7

Plasmid

#55419

Purpose

Localization: Nucleoli, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Fibrillarin (FBL Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MAPTau-C-10

Plasmid

#55431

Purpose

Localization: Microtubules, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MAPTau (MAPT Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mApple-Vinculin-23

Plasmid

#54961

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Vinculin (VCL Human)

Tags

mApple

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Expression

Bacterial

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

Plasmid

#117411

Purpose

AsCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

AsCas12a gRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119065

Purpose

Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Tags

HA

Expression

Bacterial

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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