We narrowed to 162,309 results for: addgene

1. pHPyV6-607a

   Plasmid

   #24727

   Depositor

   Christopher Buck

   Article

   Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

   Insert

   Full genome of Human polyomavirus 6
   

   Use

   Viral clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUG+p97W (C2)

   Plasmid

   #74018

   Purpose

   lentiviral expression of EGFP-SAP97beta fusion

   Depositor

   Robert Malenka, Weifeng Xu, Oliver Schluter

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 beta isoform
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB4280

   Plasmid

   #98699

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZAK (ZAKA-c057)

   Plasmid

   #98225

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ZAKA (MAP3K20 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Expression

   Mutation

   pfam00069:Pkinase 16-257

   Promoter

   polyhedrin

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-LSD1-K661A-Hygro

   Plasmid

   #104414

   Purpose

   transient expression of dCas9-dLSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dLSD1 (KDM1A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATG5 sgRNA

   Plasmid

   #207555

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   AACTTGTTTCACGCTATATC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HTATIP (2OU2)

   Plasmid

   #33338

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HTATIP (KAT5 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pCRISPR_eGFP_497

   Plasmid

   #111824

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 477-497
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ULK1 KO sgRNA

    Plasmid

    #207561

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCCGCCTGCGCCATGGAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe NEO Venus

    Plasmid

    #98396

    Purpose

    Retroviral vector for constitutive Venus expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Id2 Ngfr

    Plasmid

    #80131

    Purpose

    overexpression of ID2

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    Id2 (Id2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-Ngfr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pI6-MTU-DO-URA

    Plasmid

    #160030

    Purpose

    Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEVY-GA

    Plasmid

    #51227

    Purpose

    bi-directional expression vector for yeast, galactose inducible, ADE2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG306-GPD-AVT1 chr I

    Plasmid

    #41898

    Purpose

    Yeast expression of AVT1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    AVT1 (AVT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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