We narrowed to 157,100 results for: Des

1. pLenti CMVtight Blast DEST (w762-1)

   Plasmid

   #26434

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Lentiviral; Tet-on advanced destination vector

   Expression

   Mammalian

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST CDC42EP1 S192A

   Plasmid

   #69820

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S192A

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMVtight Hygro DEST (w769-1)

   Plasmid

   #26433

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Lentiviral; Tet-on advanced destination vector

   Expression

   Mammalian

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5/FRT/DEST-3xHA/mGli2

   Plasmid

   #51250

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

   Plasmid

   #51251

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 789,805,817,848,923,956 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-Dest-pA-pgk-bsd

   Plasmid

   #74918

   Purpose

   Gateway destination piggyBac vector

   Depositor

   Jose Silva

   Article

   dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDEST-Spe4-RfA-mScarlet-I-stop

   Plasmid

   #186393

   Purpose

   Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-DEST-hPROX1-tdTomato-PuroTK

   Plasmid

   #141027

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   tdTomato
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEF5-FRT-V5-DEST-GFP-Kif26b

    Plasmid

    #102862

    Purpose

    Expresses GFP-Kif26b in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2-actinb-kcna1a-IRES-EGFP

    Plasmid

    #164965

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcna1a (kcna1a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-CMV GFP-LC3B G120A

    Plasmid

    #123115

    Purpose

    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF5/FRT-DEST EVC2-Flag

    Plasmid

    #41005

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pDEST-CMV-N-Tandem-mCherry-EGFP

    Plasmid

    #123216

    Purpose

    N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST mfap4:icre-p2a-tomato

    Plasmid

    #135217

    Purpose

    Cre coexpressed with tdTomato in macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter with iCre and Tomato
    

    Use

    Cre/Lox; Zebrafish expression

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2/DEST/hCx43-E2HA-stop

    Plasmid

    #40909

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 internal HA tag (GJA1 Human)
    

    Expression

    Mammalian

    Mutation

    HA tag inserted in second extracellular loop

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid

    #139787

    Purpose

    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman, Jeannie Lee

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    April 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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