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pJF093

Plasmid

#113323

Purpose

Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBA2675

Plasmid

#189997

Purpose

Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Inserts

FBP75
VhhGFP4

Use

Expression vector for trypanosoma brucei

Tags

NLS

Mutation

N-terminal 600 bp of FBP75

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pSP561

Plasmid

#139497

Purpose

Expression vector for the MS2-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 MS2-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179027

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p133 pSV-STOP-luc

Plasmid

#8390

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed STOP

Use

Cre/Lox and Luciferase

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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eNOS S1179A

Plasmid

#22485

Depositor

William Sessa

Article

Fulton et al Nature. 1999 Jun 10. 399(6736):597-601.

Insert

eNOS S1179A

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pMito-iRFP720

Plasmid

#45464

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNM3814

Plasmid

#154122

Purpose

mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::rtetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

TEF

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3724

Plasmid

#154112

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRha-rsw-EYFP

Plasmid

#182928

Purpose

EYFP driven by inducible promoter Prha-rsw

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

EYFP

Promoter

Prha-rsw

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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