We narrowed to 24,551 results for: p

1. pRSET-iGluSnFR4s

   Plasmid

   #234443

   Purpose

   Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s
   

   Tags

   6xHis-Xpress epi

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti HsATP13A2 WT

   Plasmid

   #171485

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   D508N , D962N

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2-sgBAP1-2

   Plasmid

   #125838

   Purpose

   KO BAP1 gene

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti HsATP13A2 D962N

   Plasmid

   #171821

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   WT, D508N

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDule-3-nitroTyrosine (A7)

   Plasmid

   #174078

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

   Plasmid

   #71829

   Purpose

   Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLINC-AKAR1-CAAX

   Plasmid

   #87705

   Purpose

   Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FLINC-AKAR1-CAAX
   

   Tags

   C-terminal targeting sequence from Kras

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKrox24(MapERK)d1EGFP (KroxDs)

   Plasmid

   #214912

   Purpose

   for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   d1EGFP under the MapERK promoter
   

   Expression

   Mammalian

   Promoter

   MapkERK promoter

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGL4-4X Mut Renilla

   Plasmid

   #70040

   Purpose

   Control plasmid for testing 5'UTR folding elements

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   Random sequence matched for GC-content
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. 10_T7-SP-CD8tm-mScarlet3

    Plasmid

    #225937

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    SP-CD8tm-mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-M-Flag-BAP1-H169Q

    Plasmid

    #125847

    Purpose

    express mutant BAP1-H169Q

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    H169Q

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTarget_AT1R-tTA

    Plasmid

    #222851

    Purpose

    Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Tags

    FLAG and tTA

    Expression

    Mammalian

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRCN0000072225

    Plasmid

    #78160

    Purpose

    shLacZ control

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    LacZ
    

    Use

    Lentiviral and RNAi

    Promoter

    hU6 and hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-f:PGC1-3D

    Plasmid

    #47

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Amino acids 262, 265, and 298 have been changed t…

    Available Since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pBGE3.0

    Plasmid

    #165988

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRCN0000072254

    Plasmid

    #78161

    Purpose

    Luciferase control

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    Luciferase
    

    Use

    RNAi

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lyn-FLINC-EKAR1

    Plasmid

    #87707

    Purpose

    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Lyn-FLINC-EKAR1
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-CDreV

    Plasmid

    #140133

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CDreV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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