178,101 results

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pKHR4

Plasmid

#74592

Purpose

an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.

Depositor

David Grunwald

Publication

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Type

Empty backbone

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PXL

Plasmid

#75349

Purpose

PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

Depositor

Bryan Luikart

Publication

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Publication

Di Donato et al Genome Res. 2016 May;26(5):681-92. doi:

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGG-9.47_VP2_AS #2562

Plasmid

#74238

Purpose

Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Publication

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP2-AS

Use

AAV

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-Flag-CDK8-WT-IRES-mCherry

Plasmid

#68856

Purpose

Expresses Human CDK8 Wild-Type

Depositor

Matthew Shair

Publication

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Publication

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Promoter

MSCV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDH-CB-IRES-copGFP-T2A-Puro

Plasmid

#72299

Purpose

Express gene of interest under CB promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

chicken beta-actin + CMV enhancer

Available since

Jan. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pHEE401

Plasmid

#71286

Purpose

Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Publication

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Inserts

sgRNA scaffold
zCas9

Use

CRISPR; Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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