165,947 results

1. CNR1-Tango

   Plasmid

   #66254

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CNR1 (CNR1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pIF1079

   Plasmid

   #237445

   Purpose

   Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

   Depositor

   IIya Finkelstein

   Article

   Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

   Inserts

   SpCas9
   Efe1-RT
   EMX1 sgRNA
   Efe1-ncRNA targeting EMX1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti NG-ABE8e_P2A_GFP + PuroR

   Plasmid

   #242000

   Purpose

   Lentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.

   Depositor

   Frank Buchholz

   Article

   Wang et al Genome Biol. 2025 Jul 22;26(1):217. doi:

   Insert

   NG-ABE8e
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK1126

   Plasmid

   #236187

   Purpose

   BsaI compatible gentamicin resistance cassette

   Depositor

   Jeffrey Barrick

   Article

   VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

   Insert

   gentamicin resistance cassette
   

   Expression

   Bacterial

   Promoter

   CP25

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. ePop

   Plasmid

   #50953

   Purpose

   Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

   Depositor

   Lingchong You

   Article

   Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

   Inserts

   E lysis protein
   LuxI
   LuxR
   

   Mutation

   Early stop codon introduced in mutated luxR resul…

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. ppyCAG_RNaseH1_WKKD

   Plasmid

   #111905

   Purpose

   Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MLM3636

   Plasmid

   #43860

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

   Depositor

   Keith Joung

   Insert

   None
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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