We narrowed to 5,311 results for: cycs

1. 6xHis-TEV-UBC-9

   Plasmid

   #87055

   Purpose

   Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

   Depositor

   Ronald Hay

   Article

   Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

   Insert

   UBC-9 (ubc-9 Nematode)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL2-50bp

   Plasmid

   #16295

   Depositor

   Wafik El-Deiry

   Article

   Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

   Insert

   p53 promoter fragment (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   fragment -38 to +12 relative to first transcripti…

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR29

   Plasmid

   #69255

   Purpose

   pelt-2::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

   Promoter

   elt-2

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLTR-FP-GFP

   Plasmid

   #58242

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   attR1-Cm(R)-CcdB-attR2
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-G1mCNPRS

   Plasmid

   #174718

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc-ASPM CTR

   Plasmid

   #27669

   Depositor

   Joseph Loturco

   Article

   Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

   Insert

   Abnormal spindle-like microcephaly associated (Aspm Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Contains carboxy terminal region of ASPM (amino a…

   Promoter

   Available since

   March 21, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCGN-HCF-1-N1011

   Plasmid

   #92320

   Purpose

   N-term 1-1011 of HCF-1 protein

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

   Insert

   HCF-1 (HCFC1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AA 2-1011

   Promoter

   CMV

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSFG-TFII-I-GFP delta

   Plasmid

   #22190

   Depositor

   Ananda Roy

   Article

   Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPO635

   Plasmid

   #195297

   Purpose

   pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABC operon of McCAST (Tn7575)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG inducible lac promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid

    #133316

    Purpose

    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPU-Empty-miR30-TRIM5

    Plasmid

    #132936

    Purpose

    All-in-one control rescue-TRIM5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    control shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-p27(kip1)

    Plasmid

    #50026

    Purpose

    pENTR vector for human cyclin-dependent kinase inhibitor 1B

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    p27(kip1) (CDKN1B Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pOPO636

    Plasmid

    #195299

    Purpose

    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABCQ operon of McCAST (Tn7575)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG inducible lac promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. px458_2A_GFP_sgRNA_PRKRA

    Plasmid

    #106109

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting PRKRA (PRKRA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1274

    Plasmid

    #119266

    Purpose

    B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    B. subtilis deltaTn7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1273

    Plasmid

    #119265

    Purpose

    E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    E. coli attTn7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1263

    Plasmid

    #119264

    Purpose

    B. subtilis attTn7 site and flanking sequence cloned into pACYC

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    B. subtilis attTn7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p28atDarpp

    Plasmid

    #108318

    Purpose

    Expresses tDarpp in E.coli

    Depositor

    Jamil Momand

    Article

    Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

    Insert

    tDarpp (PPP1R1B Human)
    

    Use

    Tags

    6 Histadine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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