We narrowed to 6,345 results for: sas

1. pLifeAct-HyPer7

   Plasmid

   #136464

   Purpose

   Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-sACE2(WT)-Fc(IgG1)

   Plasmid

   #145163

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3-myr-AKT-HA

   Plasmid

   #31789

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

   Plasmid

   #234846

   Purpose

   3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

   Depositor

   Malin Parmar

   Article

   Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-TetON-GFP

   Plasmid

   #115495

   Purpose

   Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP-hPGK-Blast-2A-STING-TurboID

   Plasmid

   #204713

   Purpose

   Expresses C-terminal TurboID tagged human STING for proximity ligation.

   Depositor

   Nir Hacohen

   Article

   Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

   Insert

   STING1 (STING1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4 HisMax-V5-GFP-RRBP1

   Plasmid

   #92150

   Purpose

   Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 (RRBP1 Human)
   

   Use

   Tags

   Calreticulin signal peptide, EGFP (A207K mutation…

   Expression

   Mammalian

   Mutation

   In RRBP1: N951S and T1329L. E1279 is also missing.

   Promoter

   CMV

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-N-FLAG-HA-TBK1

    Plasmid

    #131791

    Purpose

    Lentiviral vector expressing WT TBK1

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    N-FLAG-HA

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCVL-UCOE-SFFV-TLR-MCV1

    Plasmid

    #117111

    Purpose

    Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    UCOE SFFV Traffic Light Reporter 2.0
    

    Use

    Lentiviral; Reporter

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid

    #178209

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HA and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid

    #178211

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear Localization Signal and Ollas.S.V5

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Use

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD649-HAsp-NEO1-COMP5AP-AviTag-9xHis

    Plasmid

    #157594

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NEO1 (NEO1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEcRNAP6

    Plasmid

    #128940

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    PreScission protease site (GE Healthcare; LEVLFQG…

    Promoter

    T7

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TBK1-C-FLAG-HA

    Plasmid

    #131792

    Purpose

    Lentiviral vector expressing WT TBK1 containing STOP codon

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    STOP codon

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-UbC-Blast-2A-STING-mNeonGreen

    Plasmid

    #227185

    Purpose

    Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

    Depositor

    Nir Hacohen

    Article

    Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

    Insert

    STING1 (STING1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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