Showing: 81 - 100 of 6921 results
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Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpGRR-r11-r10AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM690
Plasmid#74638Purposealias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM691
Plasmid#74641Purposealias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM701
Plasmid#74642Purposealias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pIM23
Plasmid#74643PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25AvailabilityAcademic Institutions and Nonprofits only -
pIM35
Plasmid#74644PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
UseTagsExpressionYeastMutationPromoterGAL1 promoter and hybrid dual-mode promoter-see c…AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
UseTagsExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 6921 results