Addgene

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

New search

Keep filters

12,747 results

Showing: 181 - 200 of 12747 results

Results per page:

SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-ST1-B

Plasmid

#48662

Purpose

Bacterial ST1 repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-ST1-A

Plasmid

#48665

Purpose

Bacterial ST1 repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer A/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

proC and tracrRNA promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
M-SPcas

Plasmid

#48668

Purpose

Mammalian S. pyogenes Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
M-NMcas

Plasmid

#48670

Purpose

Mammalian N. meningitidis Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
M-SP-sgRNA

Plasmid

#48671

Purpose

Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

Enlarge
M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU

Availability

Academic Institutions and Nonprofits only

Enlarge
M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

Enlarge
M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
M-tdTom-SP

Plasmid

#48677

Purpose

Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Sp6

Availability

Academic Institutions and Nonprofits only
M-tdTom-ST1

Plasmid

#48678

Purpose

Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Sp6

Availability

Academic Institutions and Nonprofits only
M-tdTom-NM

Plasmid

#48679

Purpose

Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Sp6

Availability

Academic Institutions and Nonprofits only
Nanog-2A-mCherry

Plasmid

#48680

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Nanog (Nanog Mouse)

Use

CRISPR

Tags

Cherry

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge