21,439 results

1. SpCas9-NG-SsAPOBEC3B

   Plasmid

   #198536

   Purpose

   Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-SsAPOBEC3B
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti X2 Blast DEST (w46-2)

   Plasmid

   #17391

   Purpose

   3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLminP_Luc2P_RE28

   Plasmid

   #90370

   Purpose

   TFEB - Lysosomal biogenesis gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   TFEB

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-EF1a-HIF2a dPA (P405A, P531A)

   Plasmid

   #232954

   Purpose

   Stably express a constitutively stable version of HIF2a (HIF2a dPA)

   Depositor

   William Kaelin

   Article

   Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

   Insert

   HIF2a (EPAS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P405A, P531A

   Promoter

   EF1a

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-UCOE-SFFV-Zim3-dCas9-BFP

   Plasmid

   #188776

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-TetOne-Puro-MCL1

   Plasmid

   #202663

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TATA-GFP

   Plasmid

   #110833

   Purpose

   negative control

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUltra-Hot-PC

   Plasmid

   #184466

   Purpose

   Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Pyruvate Carboxylase (PC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tetO-GATA6

    Plasmid

    #170688

    Purpose

    doxycycline-inducible overexpression of GATA6

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR_PGK_LaG17-mCherry-HOTag3

    Plasmid

    #190024

    Purpose

    Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    LaG17 CluMPS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PGKp-GFP-YAP (S94A)

    Plasmid

    #174173

    Purpose

    Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Serine 94 mutated to Alanine

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pFUW-tetO-GATA2

    Plasmid

    #125028

    Purpose

    doxycycline-inducible expression of human GATA2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    GATA binding protein 2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF0922

    Plasmid

    #142207

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2F1 (NR2F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX304-VHL-IRES-Tdtomato

    Plasmid

    #187643

    Purpose

    VHL Expression vector with Tdtomato

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    VHL (VHL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    UBC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BCL2alpha-MJK2243

    Plasmid

    #227690

    Purpose

    Expresses human BCL2alpha

    Depositor

    Curt Civin

    Article

    Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

    Insert

    human BCL-2alpha (BCL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF2962

    Plasmid

    #144438

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2C2 (NR2C2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge