Showing: 81 - 100 of 120 results
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Plasmid#72121PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2
Plasmid#86610PurposeExpresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmidDepositorInsertATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA
Plasmid#86612PurposeVector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
Plasmid#83842PurposeFluorescent probe for S/G2 cell cycle transition and G2/M transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7AvailabilityAcademic Institutions and Nonprofits only -
pCS2+CyclinB1-GFP
Plasmid#128426PurposeContains insert allowing in-vivo visualization of CyclinB1 protein (G2/M)DepositorInsertCyclinB1-GFP
UseTagsExpressionMammalianMutationPromoterCMV & SP6AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Vx3(A7)
Plasmid#35526DepositorInsertVx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pOC2 CRISPIE-Halo-Homer1 KI
Plasmid#183446PurposeCreON knock-in for Halo-Homer1 (amino acid position: G2)DepositorInsertgRNA and CRISPIE Halo donor
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1-His6
Plasmid#69109PurposeFor bacterial expression of recombinant human GTSE1 tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
IRES-GFP FoxG1
Plasmid#14935DepositorInsertFoxG1 (FOXG1 Human)
UseTagsIRES-GFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF-miRFP670v1-hGem(1/110)
Plasmid#80006PurposehGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)DepositorInserthGeminin (1/110) (GMNN Human)
UseLentiviralTagsmiRFP670v1ExpressionMutationPromoterEF-1 alphaAvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1ExpressionMutationcodon optimized for humanPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDP1.2
Plasmid#171092PurposeExpresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)DepositorInsertAAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
UseSynthetic BiologyTagsEmerald, GemininExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 120 results