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pAGM9121_pAOX1

Plasmid

#160912

Purpose

Alcohol oxidase 1 (AOX1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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BB1_12_pAOX1

Plasmid

#98514

Purpose

BB1 with insert: promoter PAOX1

Depositor

Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

AOX1 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK001-2-pAOX1

Plasmid

#84960

Purpose

Type 2, pAOX1, Alcohol oxidase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK019-4-tAOX1

Plasmid

#84988

Purpose

Type 4, tAOX, Alcohol oxidase 1 terminator

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pZ_P-AOX1-eGFP

Plasmid

#126718

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-NpgA

Plasmid

#126731

Purpose

yeast plasmid overexpressing NpgA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

npgA

Use

Tags

6xHis

Expression

Yeast

Mutation

G951A,C957A

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-CPR

Plasmid

#126742

Purpose

yeast plasmid overexpressing CPR

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

cpr

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-sLovA

Plasmid

#126741

Purpose

yeast plasmid overexpressing sLovA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-LovC

Plasmid

#126729

Purpose

yeast plasmid overexpressing LovC

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

lovC

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-LovB

Plasmid

#126728

Purpose

yeast plasmid overexpressing LovB

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

lovB

Use

Tags

6xHis

Expression

Yeast

Mutation

T7227A,T8994A

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pK_lacO-cP-AOX1-sAR

Plasmid

#126743

Purpose

yeast plasmid overexpressing CPR and sLovA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pK_lacO-cP-AOX1-AtX+NpgA

Plasmid

#126727

Purpose

yeast plasmid overexpressing AtX and NpgA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

atX,npgA

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pK_P-GAP-LM_lacO-cP-AOX1-sAR

Plasmid

#126745

Purpose

yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pK_P-ICL1-LM_lacO-cP-AOX1-sAR

Plasmid

#126744

Purpose

yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

ICL1

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXC3GH

Plasmid

#49023

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMJA149

Plasmid

#128524

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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