Showing: 21 - 40 of 42 results
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Plasmid#169475PurposeSingle p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAPDepositorInsertHaloTAG mGFAP (R236H) (Gfap Mouse)
UseTagsEGFPExpressionMammalianMutationChanged R 236 to HPromoterAvailabilityAcademic Institutions and Nonprofits only -
hGFAP R239C
Plasmid#169476PurposeSingle p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAPDepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationChanged R 239 to CPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorInsertPPARG (PPARG Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
PPRE-H2B-eGFP
Plasmid#84393PurposeFluorescent reporter for PPARgamma activityDepositorInsertPPARG response element (PPARG Human)
UseTagseGFPExpressionMammalianMutationPromoterPPREAvailabilityAcademic Institutions and Nonprofits only -
PPRE-pNL1.3
Plasmid#84394PurposeSecreted Luciferase reporter for PPARgamma activityDepositorInsertPPARG response element (PPARG Human)
UseTagssecNlucExpressionMammalianMutationPromoterPPREAvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-RFP-ATG9A
Plasmid#60609PurposeExpresses RFP-hATG9A in mammalian cellsDepositorInsertHomo sapiens autophagy related 9A (PPARG Human)
UseRetroviralTagsRFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-hATG9A
Plasmid#58995PurposeExpresses SECFP-hATG9A in mammalian cellsDepositorInsertHomo sapiens autophagy related 9A (PPARG Human)
UseRetroviralTagsVenusExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-R244Q
Plasmid#109023Purposestable lentiviral expression of cDNADepositorInsertATL2-R244Q (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationArg244Gln mutation results in dimerization defectPromoterEIF-1a promoterAvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα RK
Plasmid#59920PurposeExpresses mouse interleukin-2 receptor alpha chain mutatedDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationchanged sequence of the intracellular (C-terminal…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK512
Plasmid#71575PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges arginine-228 to glutamatePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
nPPARg429-gs10-NLS-VPR
Plasmid#188266PurposePlasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnPPARg429 (PPARG Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
UseTagsExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
UseTagsExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)
Plasmid#38262DepositorInsertβ-arrestin2 (Arrb2 Rat)
UseTagsFlag and PIP5K Iα core kinase domain (residues 18…ExpressionMammalianMutationArg 233, Arg237, and Lys251 converted to Glutamin…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TFORF3138
Plasmid#144614PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARG (PPARG Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF3139
Plasmid#144615PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARG (PPARG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF0885
Plasmid#142210PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARG (PPARG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCDF-Az
Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
UseTagsExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 42 results