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Plasmid#91913PurposeExpresses CaCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertCaCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-ccDockerin
Plasmid#72200PurposeMBP-ccDockerin expression plasmidDepositorInsertClostridium Cellulolyticum Type I Dockerin
UseTagsHis-Tag and Maltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pNICKclos2.0
Plasmid#73228PurposeGenome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052DepositorInsertsCas9 nickase
sgRNA to xylR
UseE.coli - clostridium shuttle vectorTagsExpressionMutationD10APromoterPj23119 and PthlAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCBEclos-cbei2385-g2-opt
Plasmid#118215PurposeCRISPR-mediated base editing in ClostridiumDepositorInsertApobec1, Casp nickase, UG1-sgRNA
UseTagsExpressionBacterialMutationPromoterPtmAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXY1-spc
Plasmid#90488PurposePlasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl
Plasmid#90485PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXY1
Plasmid#90487PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWJ1
Plasmid#90484PurposeFor gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorInsertpIM13 Origin
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Padc
Plasmid#90486PurposePlasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLT237
Plasmid#174300PurposeReplicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.DepositorInsertPolC homology arm
UseTagsExpressionBacterialMutationchanged Cysteine 669 to TyrosinePromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS1
Plasmid#174301PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS2
Plasmid#174302PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS3
Plasmid#174303PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLU_109
Plasmid#225790PurposeRS421 library variant 14073DepositorInsert14073_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_73
Plasmid#225775PurposeRS421 library variant 14073DepositorInsert14073_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_91
Plasmid#225805PurposeRS421 library variant 14073DepositorInsert14073_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only