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Showing: 1 - 20 of 116 results
  1. DNMT3A

    Plasmid
    #25328
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    DNMT3A_11 (DNMT3A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. gRNA_DNMT3a-T2

    Plasmid
    #41822
    Purpose
    Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering
    Depositor
    Insert
    gRNA_DNMT3a-T2 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 116 results