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Showing: 21 - 40 of 1574 results

  2. pTRIPZ (M)-YFP-Eed

    Plasmid
    #82512
    Purpose
    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    embryonic ectoderm development
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    insert contains AA39-441 of EeD
    Promoter
    cmv
    Availability
    Academic Institutions and Nonprofits only
    82512-plasmid-map-sequence-id-171489

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  6. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Availability
    Academic Institutions and Nonprofits only

  10. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Availability
    Academic Institutions and Nonprofits only

  18. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    200402-plasmid-map-sequence-id-396788

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  19. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200403
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    200403-plasmid-map-sequence-id-396808

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  20. 3JPX

    Plasmid
    #60122
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED:APC056-C05:C32049 (EED Human)
    Use
    Tags
    6xHis and GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 1574 results