Showing: 41 - 60 of 4166 results
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Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-FBL
Plasmid#63672PurposePITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locusDepositorInsertEGFP-2A-PuroR
UseCRISPRTagsExpressionMutationPromoterPromoterlessAvailabilityAcademic Institutions and Nonprofits only -
pME-FlEx switch
Plasmid#82406PurposeGateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorInsertSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterchicken β-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #1
Plasmid#104216PurposeBacterial expression of WW domain #1 from ITCHDepositorInsertITCH WW domain #1 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #3
Plasmid#104218PurposeBacterial expression of WW domain #3 from ITCHDepositorInsertITCH WW domain #3 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #4
Plasmid#104219PurposeBacterial expression of WW domain #4 from ITCHDepositorInsertITCH WW domain #4 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
IL-8 toehold switch sensor
Plasmid#111908PurposeToehold switch sensor to detect IL-8 mRNA with GFP outputDepositorInsertIL8 toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CpcB-Picea-sitchensisPHLS_CpcA
Plasmid#74001PurposeExpresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in SynechocystisDepositorInsertPHLS and CmR
UseTagsExpressionMutationPromotercpcAvailabilityAcademic Institutions and Nonprofits only -
pUC19-Mu-switch-R-loop
Plasmid#134899PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmouse IgH switch repeat region
UseSynthetic BiologyTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ACTB
Plasmid#207748PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of ACTB (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H2BC11
Plasmid#207755PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBA1B
Plasmid#207763PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-LMNB1
Plasmid#207770PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H3C2
Plasmid#207780PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of H3C2 (H3C2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBB4B
Plasmid#207785PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-GOLGA2
Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SwitchON-sgCh2-2
Plasmid#199637PurposeTamoxifen-inducible expression of sgRNA control targeting intergenic regionDepositorInsertN/A
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 4166 results