Showing: 1 - 20 of 22 results
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Plasmid#50941Purposeexpression of full length untagged human RanGAP1 in E.coliDepositorInsertRanGTPase activating protein 1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RANGAP1 G5.4 gRNA
Plasmid#90872Purpose3rd generation lentiviral gRNA plasmid targeting human RANGAP1DepositorInsertRANGAP1 (Guide Designation G5.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
RANGAP1 G6.4 gRNA
Plasmid#90873Purpose3rd generation lentiviral gRNA plasmid targeting human RANGAP1DepositorInsertRANGAP1 (Guide Designation G6.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pET23a RanGAP
Plasmid#13387DepositorInsertRanGAP (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2 RanGAP
Plasmid#13378DepositorInsertRanGAP (RANGAP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2 RanGAP
Plasmid#13379DepositorInsertRanGAP (RANGAP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDsRed1-N1 RanGAP
Plasmid#13386DepositorInsertRanGAP (RANGAP1 Human)
UseTagsDsRed1ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterUbiquitin promoterAvailabilityAcademic Institutions and Nonprofits only -
pMTB2-RanGAP-Cerulean-Avi
Plasmid#80068PurposeBeta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseZebrafish biotaggingTagsAviExpressionBacterialMutationPromoterBeta-actin2AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
Plasmid#216248PurposeHDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSDDepositorInsertHDR template for human RANGAP1
UseCRISPR and TALEN; Hdr templateTagsminiIAA7.3xFlag.P2A.BSDExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP*
Plasmid#175237PurposeBacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3flag-RanGAPdeltaN
Plasmid#25104DepositorInsertRanGAP1 deleted N-term domain (RANGAP1 Human)
UseTagsFlagExpressionMammalianMutationDeleted amino acids 1-203.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET30 SUMO-1 G97
Plasmid#13381DepositorInsertSUMO-1, G97 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationG97 mutant: lacks 4 amino acids from the C-termin…PromoterAvailabilityAcademic Institutions and Nonprofits only -
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 22 results