We narrowed to 866 results for: seph

1. pBSMrnaSeph

   Plasmid

   #67507

   Purpose

   Express the tRNAMet scaffold with the sephadex aptamer tag

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet with sephadex aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBSKrnaSeph

   Plasmid

   #67506

   Purpose

   Express the tRNALys scaffold with the sephadex aptamer tag

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNALys with sephadex aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. mCherry-SEpHluorin

   Plasmid

   #32001

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   mCherry
   

   Use

   Tags

   Superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNb-SEpHluorin

   Plasmid

   #128785

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   RNb-SEpHluorin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SEPHS2 gRNA (BRDN0001148921)

   Plasmid

   #77661

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SEPHS2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SEPHS2 (SEPHS2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SEPHS2 gRNA (BRDN0001146226)

   Plasmid

   #77662

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SEPHS2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SEPHS2 (SEPHS2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SEPHS2 gRNA (BRDN0001147864)

   Plasmid

   #77663

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SEPHS2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SEPHS2 (SEPHS2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SEPHS2 gRNA (BRDN0001149011)

   Plasmid

   #77664

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SEPHS2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SEPHS2 (SEPHS2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Use

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Lyn-tailed mCherry-SEpHluorin

    Plasmid

    #32002

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    Membrane-targeting sequence of Lyn kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEXP1/Joseph2-Multitagged Plasmid

    Plasmid

    #226724

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pEXP1/Joseph2
    

    Use

    Tags

    6xHis, HA, Myc, GFP, Flag, V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAHW/Joseph2-Multitagged Plasmid

    Plasmid

    #226725

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pAHW/Joseph2
    

    Use

    Drosophila s2 cell expression

    Tags

    HA, Myc, GFP, Flag, V5, 6xHis

    Expression

    Insect

    Mutation

    Promoter

    Act5C

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64TNE Xnr4 (DM#174)

    Plasmid

    #15078

    Depositor

    Douglas Melton

    Article

    Joseph et al Dev Biol. 1997 Apr 15. 184(2):367-72.

    Insert

    Xnr4 (xnr4 Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. p413GPD-hTPI

    Plasmid

    #50719

    Purpose

    expression of human TPI1 in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDsRed1-N1 RanGAP

    Plasmid

    #13386

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-2 RanGAP

    Plasmid

    #13379

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pET23a RanGAP

    Plasmid

    #13387

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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