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Addgene
Showing: 61 - 80 of 385 results

  1. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  3. pCMVβ-p300.DY-myc

    Plasmid
    #30490
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    aspartic acid 1399 converted to tyrosine (Acetyla…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  4. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  5. PP-PCMT

    Plasmid
    #25106
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0309:C34112
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. pJK644

    Plasmid
    #71629
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71629-plasmid-map-sequence-id-131648

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  7. pJK608

    Plasmid
    #71621
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to aspartate, additional N-term…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71621-plasmid-map-sequence-id-131639

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  8. pJK655

    Plasmid
    #71632
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate, phenylalanine-79 …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71632-plasmid-map-sequence-id-131652

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  9. GARS_pNIC-Bio3

    Plasmid
    #153049
    Purpose
    Expression construct for producing Avi tagged Aspartyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    GARS (GARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153049-plasmid-map-sequence-id-304463

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  11. pLenti4/V5-DEST-P85(D560Y)

    Plasmid
    #40223
    Depositor
    Lynda Chin
    Insert
    P85(D560Y) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 560 to Tyrosine
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  12. DNPEP

    Plasmid
    #39040
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DNPEP (DNPEP Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. (PM-S608D)FLAG-Cep215-FL

    Plasmid
    #106909
    Purpose
    Expresses murine phospho-mimetic CEP215 at S608
    Depositor
    Heidi Hehnly
    Insert
    CEP215 (Cdk5rap2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed Serine-608 to Aspartic Acid
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    106909-plasmid-map-sequence-id-204099

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  14. APPL1-N308D-GFP

    Plasmid
    #102783
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    102783-plasmid-map-sequence-id-198005

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  15. APPL1-N308D-mCherry

    Plasmid
    #102784
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    102784-plasmid-map-sequence-id-197993

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  16. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

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  18. GOT1

    Plasmid
    #39022
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GOT1 (GOT1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  19. FUW-hSNCA(D2R)-NE

    Plasmid
    #215487
    Purpose
    Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells
    Depositor
    Ophir Shalem
    Insert
    alpha-synuclein (SNCA Human)
    Use
    Lentiviral
    Tags
    NE tag
    Expression
    Mammalian
    Mutation
    D (second aa) mutated to R
    Promoter
    Ubc
    Availability
    Academic Institutions and Nonprofits only
    215487-plasmid-map-sequence-id-426301

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  20. FUW-hSNCA(D2E)-NE

    Plasmid
    #215488
    Purpose
    Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells
    Depositor
    Ophir Shalem
    Insert
    alpha-synuclein (SNCA Human)
    Use
    Lentiviral
    Tags
    NE tag
    Expression
    Mammalian
    Mutation
    D (second aa) mutated to E
    Promoter
    Ubc
    Availability
    Academic Institutions and Nonprofits only
    215488-plasmid-map-sequence-id-426309

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Showing: 61 - 80 of 385 results