Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1 - 19 of 19 results

  2. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

    Enlarge

  4. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

    Enlarge

  5. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid
    #69115
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of 678-681aa region. Silent mutations i…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69115-plasmid-map-sequence-id-122094

    Enlarge

  6. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid
    #69116
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 682-688 (region containin…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69116-plasmid-map-sequence-id-122088

    Enlarge

  11. Adeno FT

    Plasmid
    #101824
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    U6 and CBh
    Availability
    Academic Institutions and Nonprofits only

  18. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid
    #69113
    Purpose
    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to confer shRNA resistance.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69113-plasmid-map-sequence-id-121841

    Enlarge

  19. pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

    Plasmid
    #69114
    Purpose
    Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    L1939A and L1942A amino acid substitutions. Sile…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    69114-plasmid-map-sequence-id-122082

    Enlarge

Showing: 1 - 19 of 19 results